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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has been noticed in many tumors, which ends up in chromosome instability, and thus may perhaps contribute to tumorigenesis. This protein was discovered to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a vital position in advertising and marketing mobile-cycle progression inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, happen to be explained. Two added splice variants have been claimed but in depth nucleotide sequence information will not be yet offered.
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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide normally incorporates the epitope identified via the antibody.
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This tutorial outlines more possible optimization of cellular and immunoassay parameters to make certain the best possible results are...
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This guide outlines even further possible optimization of cellular and immunoassay parameters to guarantee the absolute best results are...
Antibodies certain to the blocking peptide now not bind to your epitope around the concentrate on protein. By evaluating the staining from the blocked antibody as opposed to the antibody by itself, you can see which staining is particular.
Goal Information Cyclin E belongs to your extremely conserved cyclin household, whose members are characterized by a extraordinary periodicity in protein abundance with the mobile cycle. Cyclins functionality as regulators of CDK kinases. Distinct cyclins show distinctive expression and degradation patterns which add into the temporal coordination of each and every mitotic celebration. Cyclin E kinds a fancy with and functions to be a regulatory subunit of CDK2, whose exercise is needed for mobile cycle G1/S changeover. This protein accumulates in the G1-S period boundary and is particularly degraded as cells development by means of S stage.
The measurement of protein phosphorylation is read more a useful gizmo for measuring the modulation of receptor activation by the two antibodies...
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